Protein and antibody purification followed by immunoprecipitation of MYB and GATA zinc finger-type maize proteins with magnetic beads.

Co-immunoprecipitation (Co-IP) is a widely used and powerful approach for studying protein-protein interactions in vivo. Here, we describe a protocol for antibody purification and immobilization followed by immunoprecipitation from plant tissue extracts using magnetic beads. The protocol has been used to detect regulators in the Zea mays phenylpropanoid pathway. The protocol is amenable to a variety of downstream assays, including western blotting and mass spectrometry. For complete details on the use and execution of this protocol, please refer to Vélez-Bermúdez et al. (2015).

Efectos de la infección y la enfermedad por citomegalovirus en receptores de trasplante renal / Effects of cytomegalovirus infection and disease in renal transplant recipients

La infección por citomegalovirus (CMV) es una de las más importantes causas de morbilidad en pacientes con trasplante renal. Objetivo: revisar las manifestaciones de la enfermedad aguda por este virus y sus efectos directos e indirectos sobre injerto y receptor en pacientes con trasplante renal. Material y métodos: se hizo una revisión sistemática de la literatura en la que se encontraron 40 publicaciones completas relacionadas con el tema. Conclusiones: además de la enfermedad aguda que se manifiesta con síndrome de mononucleosis o daño tisular, el CMV produce efectos indirectos sobre el injerto y el sistema vascular del receptor que parecen aumentar el riesgo de enfermedad cardiovascular y acortan la supervivencia del trasplante y el receptor. Las estrategias utilizadas en los últimos años han logrado disminuir el efecto nocivo de la enfermedad aguda por CMV, pero se desconoce su impacto sobre los efectos indirectos de la infección latente.(Acta Med Colomb 2012; 37: 131-137).

Citomegalovirus infection (CMV) is one of the major causes of morbidity in renal transplant patients. Objective: to review the manifestations of the acute viral illness and its direct and indirect effects on graft and recipient in transplant patients. Material and methods: we conducted a systematic review of the literature in which we found 40 complete publications related to the topic. Conclusions: in addition to the acute illness that manifests with mononucleosis syndrome or tissue damage, CMV has indirect effects on the graft and the receptor’s vascular system that appear to increase the risk of cardiovascular disease and shorten both graft and receiver survival. The strategies used in recent years have reduced the deleterious effect of acute CMV disease, but its impact on the indirect effects of the latent infection is unknown. (Acta Med Colomb 2012; 37: 131-137).